Review



ncode mirna first-strand cdna synthesis mirc-50 kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Thermo Fisher ncode mirna first-strand cdna synthesis mirc-50 kit
    Ncode Mirna First Strand Cdna Synthesis Mirc 50 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/us11993816-492-20-19
    Average 90 stars, based on 1 article reviews
    ncode mirna first-strand cdna synthesis mirc-50 kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Reverse Transcription:

    Article Title: Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA
    Article Snippet: Total RNA was extracted by TRIzol reagent (Invitrogen, Grand Island, NY, USA), and the quality of RNA was evaluated by the standard method . .. A sample of RNA (500 ng) was reverse transcribed to cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). qPCR was performed using the FastStart Universal SYBR Green Master (Roche, Mannheim, Germany) with the universal reverse primer provided in the kit and the prepared cDNA as the template. qPCR was performed on the Applied Biosystems real-time detection system (Applied Biosystems, Foster City, CA, USA) using the following thermocycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s followed by 60°C for 30 s. Each sample was run in triplicate, and expression levels were normalized against U6 snRNA levels [U6: 5′-CTTCGGCAGCACATATACT-3′ (forward) and 5′-AAAATATGGAACGCTTC ACG-3′ (reverse)]. ..

    Article Title: Chicken miR-148a-3p regulates immune responses against AIV by targeting the MAPK signalling pathway and IFN-γ
    Article Snippet: A universal primer from the NcodeTM miRNA First-Strand cDNA Synthesis Kit (Invitrogen) was used as the reverse primer. .. The total RNA was reverse-transcribed into cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Quantitative PCR was performed using AMPIGENE® qPCR Green Mix Lo-ROX (Enzo Life Sciences) in 45 cycles of incubation at 95 °C for 15 s and 55 °C for 30 s in a CFX Connect Real-time PCR (Bio-Rad) device according to the manufacturer’s instructions.

    Article Title: MicroRNA-650 Regulates the Pathogenesis of Alzheimer’s Disease Through Targeting Cyclin-Dependent Kinase 5
    Article Snippet: Primary/precursor miR-650 was detected by polyadenylating total RNA using SuperScript III oligo-dT reverse transcription (Invitrogen) to generate first-strand cDNA and real-time PCR targeting the pri-/pre-miR-650. .. Polyadenylation and reverse transcription were performed according to the manufacturer’s instructions using the Ncode miRNA First-Strand cDNA Synthesis kit (Invitrogen), with 1 μg of total RNA isolated with TRIZOL as input. ..

    Article Title: MicroRNA-374a Promotes Hepatocellular Carcinoma Cell Proliferation by Targeting Mitogen-Inducible Gene 6 (MIG-6)
    Article Snippet: Total cellular RNA was extracted with TRIzol reagent (Invitrogen, Grand Island, NY) according to the manufacturer’s protocol. .. RNA (500 ng) was reverse transcribed to cDNA using an NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. RT-PCR was conducted using an Applied Biosystems real-time detection system (Applied Biosystems, Darmstadt, Germany).

    cDNA Synthesis:

    Article Title: Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA
    Article Snippet: Total RNA was extracted by TRIzol reagent (Invitrogen, Grand Island, NY, USA), and the quality of RNA was evaluated by the standard method . .. A sample of RNA (500 ng) was reverse transcribed to cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). qPCR was performed using the FastStart Universal SYBR Green Master (Roche, Mannheim, Germany) with the universal reverse primer provided in the kit and the prepared cDNA as the template. qPCR was performed on the Applied Biosystems real-time detection system (Applied Biosystems, Foster City, CA, USA) using the following thermocycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s followed by 60°C for 30 s. Each sample was run in triplicate, and expression levels were normalized against U6 snRNA levels [U6: 5′-CTTCGGCAGCACATATACT-3′ (forward) and 5′-AAAATATGGAACGCTTC ACG-3′ (reverse)]. ..

    Article Title: Chicken miR-148a-3p regulates immune responses against AIV by targeting the MAPK signalling pathway and IFN-γ
    Article Snippet: A universal primer from the NcodeTM miRNA First-Strand cDNA Synthesis Kit (Invitrogen) was used as the reverse primer. .. The total RNA was reverse-transcribed into cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Quantitative PCR was performed using AMPIGENE® qPCR Green Mix Lo-ROX (Enzo Life Sciences) in 45 cycles of incubation at 95 °C for 15 s and 55 °C for 30 s in a CFX Connect Real-time PCR (Bio-Rad) device according to the manufacturer’s instructions.

    Article Title: MicroRNA-650 Regulates the Pathogenesis of Alzheimer’s Disease Through Targeting Cyclin-Dependent Kinase 5
    Article Snippet: Primary/precursor miR-650 was detected by polyadenylating total RNA using SuperScript III oligo-dT reverse transcription (Invitrogen) to generate first-strand cDNA and real-time PCR targeting the pri-/pre-miR-650. .. Polyadenylation and reverse transcription were performed according to the manufacturer’s instructions using the Ncode miRNA First-Strand cDNA Synthesis kit (Invitrogen), with 1 μg of total RNA isolated with TRIZOL as input. ..

    Article Title: MicroRNA-374a Promotes Hepatocellular Carcinoma Cell Proliferation by Targeting Mitogen-Inducible Gene 6 (MIG-6)
    Article Snippet: Total cellular RNA was extracted with TRIzol reagent (Invitrogen, Grand Island, NY) according to the manufacturer’s protocol. .. RNA (500 ng) was reverse transcribed to cDNA using an NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. RT-PCR was conducted using an Applied Biosystems real-time detection system (Applied Biosystems, Darmstadt, Germany).

    Article Title: miR-548 predicts clinical prognosis and functions as a tumor promoter in gastric cancer.
    Article Snippet: Numerous pieces of evidence have demonstrated the functional role of miR-548 in various cancers.. The expression and function of miR-548 in gastric cancer were investigated in the present study.. A total of 123 gastric cancer patients were included and provided paired gastric cancer tissues and matched normal tissues.

    Article Title: MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-Hydroxyphenyl)retinamide
    Article Snippet: .. The expression of selected miRNAs in ARPE-19 cells was verified by real-time PCR employing NCode SYBR Green miRNA qRT–PCR Kit and NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Total RNA samples (1 μg) were first treated with DNase I and then subjected to a polyadenylation reaction before first strand cDNA synthesis with SuperScript III and Universal RT Primer (Invitrogen).

    Article Title: The Mechanism of SNHG8/Microrna-421-3p/Sorting Nexin 8 Axis on Dopaminergic Neurons in Substantia Nigra in a Mouse Model of Parkinson's Disease.
    Article Snippet: Parkinson’s disease (PD) is a progressive neurodegenerative disease affecting the aging population.. Particularly, long noncoding RNAs (lncRNAs) have been demonstrated to play vital roles in PD, while the role of lncRNA SNHG8 in PD remains to be further explored.. C57BL/6 mice were induced by rotenone to establish a PD model in vivo, and then the dopaminergic (DA) neuronal damage and locomotor dysfunction in rotenone-treated mice were evaluated.

    Real-time Polymerase Chain Reaction:

    Article Title: Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA
    Article Snippet: Total RNA was extracted by TRIzol reagent (Invitrogen, Grand Island, NY, USA), and the quality of RNA was evaluated by the standard method . .. A sample of RNA (500 ng) was reverse transcribed to cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). qPCR was performed using the FastStart Universal SYBR Green Master (Roche, Mannheim, Germany) with the universal reverse primer provided in the kit and the prepared cDNA as the template. qPCR was performed on the Applied Biosystems real-time detection system (Applied Biosystems, Foster City, CA, USA) using the following thermocycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s followed by 60°C for 30 s. Each sample was run in triplicate, and expression levels were normalized against U6 snRNA levels [U6: 5′-CTTCGGCAGCACATATACT-3′ (forward) and 5′-AAAATATGGAACGCTTC ACG-3′ (reverse)]. ..

    Article Title: MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-Hydroxyphenyl)retinamide
    Article Snippet: .. The expression of selected miRNAs in ARPE-19 cells was verified by real-time PCR employing NCode SYBR Green miRNA qRT–PCR Kit and NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Total RNA samples (1 μg) were first treated with DNase I and then subjected to a polyadenylation reaction before first strand cDNA synthesis with SuperScript III and Universal RT Primer (Invitrogen).

    SYBR Green Assay:

    Article Title: Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA
    Article Snippet: Total RNA was extracted by TRIzol reagent (Invitrogen, Grand Island, NY, USA), and the quality of RNA was evaluated by the standard method . .. A sample of RNA (500 ng) was reverse transcribed to cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). qPCR was performed using the FastStart Universal SYBR Green Master (Roche, Mannheim, Germany) with the universal reverse primer provided in the kit and the prepared cDNA as the template. qPCR was performed on the Applied Biosystems real-time detection system (Applied Biosystems, Foster City, CA, USA) using the following thermocycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s followed by 60°C for 30 s. Each sample was run in triplicate, and expression levels were normalized against U6 snRNA levels [U6: 5′-CTTCGGCAGCACATATACT-3′ (forward) and 5′-AAAATATGGAACGCTTC ACG-3′ (reverse)]. ..

    Article Title: MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-Hydroxyphenyl)retinamide
    Article Snippet: .. The expression of selected miRNAs in ARPE-19 cells was verified by real-time PCR employing NCode SYBR Green miRNA qRT–PCR Kit and NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Total RNA samples (1 μg) were first treated with DNase I and then subjected to a polyadenylation reaction before first strand cDNA synthesis with SuperScript III and Universal RT Primer (Invitrogen).

    Expressing:

    Article Title: Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA
    Article Snippet: Total RNA was extracted by TRIzol reagent (Invitrogen, Grand Island, NY, USA), and the quality of RNA was evaluated by the standard method . .. A sample of RNA (500 ng) was reverse transcribed to cDNA using the NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). qPCR was performed using the FastStart Universal SYBR Green Master (Roche, Mannheim, Germany) with the universal reverse primer provided in the kit and the prepared cDNA as the template. qPCR was performed on the Applied Biosystems real-time detection system (Applied Biosystems, Foster City, CA, USA) using the following thermocycling conditions: 95°C for 3 min, 40 cycles of 95°C for 15 s followed by 60°C for 30 s. Each sample was run in triplicate, and expression levels were normalized against U6 snRNA levels [U6: 5′-CTTCGGCAGCACATATACT-3′ (forward) and 5′-AAAATATGGAACGCTTC ACG-3′ (reverse)]. ..

    Article Title: MicroRNA expression in human retinal pigment epithelial (ARPE-19) cells: Increased expression of microRNA-9 by N-(4-Hydroxyphenyl)retinamide
    Article Snippet: .. The expression of selected miRNAs in ARPE-19 cells was verified by real-time PCR employing NCode SYBR Green miRNA qRT–PCR Kit and NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen). .. Total RNA samples (1 μg) were first treated with DNase I and then subjected to a polyadenylation reaction before first strand cDNA synthesis with SuperScript III and Universal RT Primer (Invitrogen).

    Isolation:

    Article Title: MicroRNA-650 Regulates the Pathogenesis of Alzheimer’s Disease Through Targeting Cyclin-Dependent Kinase 5
    Article Snippet: Primary/precursor miR-650 was detected by polyadenylating total RNA using SuperScript III oligo-dT reverse transcription (Invitrogen) to generate first-strand cDNA and real-time PCR targeting the pri-/pre-miR-650. .. Polyadenylation and reverse transcription were performed according to the manufacturer’s instructions using the Ncode miRNA First-Strand cDNA Synthesis kit (Invitrogen), with 1 μg of total RNA isolated with TRIZOL as input. ..

    Article Title: MicroRNA-650 Regulates the Pathogenesis of Alzheimer's Disease Through Targeting Cyclin-Dependent Kinase 5.
    Article Snippet: Primary/precursor miR-650 was detected by polyadenylating total RNA using SuperScript III oligo-dT reverse 1 3 2428 Molecular Neurobiology (2023) 60:2426–2441 transcription (Invitrogen) to generate first-strand cDNA and real-time PCR targeting the pri-/pre-miR-650. .. Polyadenylation and reverse transcription were performed according to the manufacturer’s instructions using the Ncode miRNA First-Strand cDNA Synthesis kit (Invitrogen), with 1 μg of total RNA isolated with TRIZOL as input. ..

    Extraction:

    Article Title: miR-548 predicts clinical prognosis and functions as a tumor promoter in gastric cancer.
    Article Snippet: Numerous pieces of evidence have demonstrated the functional role of miR-548 in various cancers.. The expression and function of miR-548 in gastric cancer were investigated in the present study.. A total of 123 gastric cancer patients were included and provided paired gastric cancer tissues and matched normal tissues.



    Similar Products

    90
    Thermo Fisher ncode mirna first-strand cdna synthesis mirc-50 kit
    Ncode Mirna First Strand Cdna Synthesis Mirc 50 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/us11993816-492-20-19
    Average 90 stars, based on 1 article reviews
    ncode mirna first-strand cdna synthesis mirc-50 kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher ncode™ mirna first-strand cdna synthesis kit
    Ncode™ Mirna First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/pm37993949-58-5-11
    Average 90 stars, based on 1 article reviews
    ncode™ mirna first-strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher ncode mirna first-strand cdna synthesis kit
    Ncode Mirna First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/pmc10664352-42-11-15
    Average 90 stars, based on 1 article reviews
    ncode mirna first-strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher mirna reverse transcriptase ncode mirna first-strand cdna synthesis kit
    Mirna Reverse Transcriptase Ncode Mirna First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/mirna+reverse+transcriptase+ncode+mirna+first+strand+cdna+synthesis+kit/pm36840487-67-13-19
    Average 90 stars, based on 1 article reviews
    mirna reverse transcriptase ncode mirna first-strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher mirna reverse transcriptase (ncode mirna first‐strand cdna synthesis kit
    Mirna Reverse Transcriptase (Ncode Mirna First‐Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncode+mirna+first-strand+cdna+synthesis+kit/pmc09950875-57-13-19
    Average 90 stars, based on 1 article reviews
    mirna reverse transcriptase (ncode mirna first‐strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results